The Tuskegee Golden Tigers football program is the intercollegiate American football team for the Tuskegee University located in the U.S. state of Alabama. The team competes in the NCAA Division II level and are members of the Southern Intercollegiate Athletic Conference. The school's first football team was fielded in 1913. The team plays its home games at the 10,000 seat Abbott Memorial Alumni Stadium. They are coached by Willie Slater.

The Tuskegee Golden Tigers football program is the intercollegiate American football team for the Tuskegee University located in the U.S. state of Alabama.